Purdue biology innovation allows the introduction of valuable traits in plants without creating transgenic plants

WEST LAFAYETTE, Ind. – Academic researchers and companies in the agricultural biotechnology sector will be able to use a patent-pending Purdue University biology innovation to introduce valuable traits to plants without integrating novel DNA into a plant’s genome.

Stanton Gelvin (left), the Edwin Umbarger Distinguished Professor of Biology, and Lan-Ying Lee, research scientist in the Department of Biological Sciences of Purdue University’s College of Science, have developed patent-pending Agrobacterium strains that deliver T-DNA to plants but do not integrate this DNA into the plant genome. The plants can still be modified to express valued traits, but they are not transgenic. (Purdue University College of Science photo/Alisha Referda) 

 

Traditional Agrobacterium strains deliver transfer DNA, or T-DNA, into plants, including crops, and integrate it into the plants’ genome. This can create a plant that expresses traits, such as improved drought resistance or better nutritional content, that are valued by growers and can be useful to industry. However, T-DNA is permanently integrated into the plant genome, creating plants labeled “transgenic.” Transgenic plants can be either highly regulated or outlawed.

Purdue biologists have developed Agrobacterium strains that deliver T-DNA so plants can still be modified to express valued traits, but they are not transgenic. This means traditional methods to remove T-DNA aren’t needed. The strains were created by Stanton Gelvin, the Edwin Umbarger Distinguished Professor of Biology, and Lan-Ying Lee, research scientist, in the Department of Biological Sciences of Purdue University’s College of Science.

Gelvin said these VirD2 mutant Agrobacterium strains can carry T-DNA that delivers and expresses genome engineering reagents, such as CRISPR-Cas. Although the plant genome may be altered, no transgenic plant is created.

“T-DNA delivered by Purdue-created Agrobacterium strains disappears from the plant nucleus because it is eventually destroyed by nucleases – naturally existing enzymes that degrade DNA – or it is ‘diluted’ out of the plant nuclei as the cells divide,” Gelvin said.

The traditional method to remove integrated T-DNA from transgenic plants is to sexually cross a transgenic plant with a nontransgenic plant. Gelvin said this method has drawbacks.

“Crossing can be time-consuming and costly, generally requiring several generations of plants,” Gelvin said. “This isn’t feasible for plants with long generation times, such as many trees used for fruit or production of lumber, or crops that are normally vegetatively propagated, such as potatoes, sweet potatoes and bananas. These Purdue-created Agrobacterium strains avoid these drawbacks.”

Gelvin and Lee have successfully used their strains on preliminary genome engineering of model plant species. Their altered Agrobacterium strains mutated a tobacco phytoene desaturase gene, encoding an enzyme involved in chlorophyll synthesis, at 50%-80% of levels mutated by normal, wild-type Agrobacterium strains, but without generating a transgenic plant. 

“There are numerous traits that scientists and companies would like to introduce, but our strains are perhaps best used for genome engineering of any gene,” Gelvin said. “Lan-Ying and I continue to conduct additional experiments as we try to make these strains easier to use in academic laboratory and industrial settings.”

Gelvin and Lee have disclosed their Agrobacterium strains to the Purdue Research Foundation Office of Technology Commercialization, which has applied for patent protection on the intellectual property. Commercial partners with an interest in developing or licensing the strains should contact Abhijit Karve, director of business development, aakarve@prf.org, about tracking code 2023-GELV-70225.

Gelvin and Lee have received support from the National Science Foundation to develop these Agrobacterium strains.

 

About Purdue University

Purdue University is a top public research institution developing practical solutions to today’s toughest challenges. Ranked in each of the last five years as one of the 10 Most Innovative universities in the United States by U.S. News & World Report, Purdue delivers world-changing research and out-of-this-world discovery. Committed to hands-on and online, real-world learning, Purdue offers a transformative education to all. Committed to affordability and accessibility, Purdue has frozen tuition and most fees at 2012-13 levels, enabling more students than ever to graduate debt-free. See how Purdue never stops in the persistent pursuit of the next giant leap at https://stories.purdue.edu.

 

About Purdue Research Foundation Office of Technology Commercialization

The Purdue Research Foundation Office of Technology Commercialization operates one of the most comprehensive technology transfer programs among leading research universities in the U.S. Services provided by this office support the economic development initiatives of Purdue University and benefit the university’s academic activities through commercializing, licensing and protecting Purdue intellectual property. In fiscal year 2022, the office reported 157 deals finalized with 237 technologies signed, 379 disclosures received and 169 issued U.S. patents. The office is managed by the Purdue Research Foundation, which received the 2019 Innovation and Economic Prosperity Universities Award for Place from the Association of Public and Land-grant Universities. In 2020, IPWatchdog Institute ranked Purdue third nationally in startup creation and in the top 20 for patents. The Purdue Research Foundation is a private, nonprofit foundation created to advance the mission of Purdue University. Contact otcip@prf.org for more information.

 

Comments (0)

This post does not have any comments. Be the first to leave a comment below.


Post A Comment

You must be logged in before you can post a comment. Login now.

Featured Product

Get enhanced vision-powered systems for Auto Farming!

Get enhanced vision-powered systems for Auto Farming!

e-con Systems leads the way in Agriculture Automation, leveraging over 20 years of expertise in OEM camera design, development, and manufacturing. e-con's diverse camera range, featuring advanced capabilities and partnerships with leading sensor technology providers like Sony, onsemi, and OmniVision, is tailored for various smart farming applications such as weed detection, bug identification, crop health monitoring, and harvesting automation. The recent addition of e-con's 20MP camera captures tiny details, enhancing efficiency in crop monitoring and automated machinery guidance. e-con cameras offer versatile interfaces, exceptional low-light performance, expert ISP tuning, multi-camera support, HDR, long-distance capabilities, and customizable features, empowering agriculture automation. e-con specializes in providing AI-ready multi-camera solutions for real-time decision-making. e-con Systems has empowered numerous clients in the smart farming industry to develop and deploy futuristic agricultural systems, including agricultural robots, autonomous tractors, and drones. As NVIDIA's Elite partner, e-con Systems revolutionizes agricultural applications with deep learning algorithms for NVIDIA Jetson kits, expediting time-to-market for customers.